The invention discloses a method for assisting to authenticate milk cows with different milk producing characteristics and special primer pairs used by the same. The primer pairs provided by the invention are the primer pairs consisting of DNAs shown as a sequence 5 and a sequence 6 in a sequence table. The method provided by the invention comprises the following steps of: carrying out PCR (Polymerase Chain Reaction) amplification on a gene group DNA of the milk cows to be detected by using the primer pairs; and judging whether the milk cows to be detected are TT gene types or TC gene types according to a PCR product. The milk producing characteristics of the TC gene type milk cows are superior to those of the TT gene type milk cows and comprise high milk producing amount and/or high lactoprotein amount. In the invention, the provided method has the advantages of convenient use and high sensitivity, and can be applied to early selection and grouping of milk cows with high yield and mammitis resistance in Chinese Holstein cattle. The invention provides theoretical basis and hereditary basis for culturing molecular breeding of milk cows with high yield and mammitis resistance, is beneficial to quickening the breeding progress of the milk cows, and has great important application values and social benefits.