The invention relates to construction and application of a recombined lentivirus vector aiming at RNA interference of PKC gamma genes. The construction comprises the following steps of: experimentally constructing the lentivirus vector of ShRNA aiming at the PKC gamma genes, mediating the synthetic DNA fragments aiming at ShRNA by the lentivirus vector, performing cotransfection of 293T cells with two kinds of vectors of pHelper 1.0 and pHelper 2.0 and then culturing the cells, and performing transfection of target cells after the lentivirus vector is recombined to realize the RNA interference aiming at the PKC gamma genes. Because the third generation lentivirus vector (SIN) which is self-inactivated is used, the recombined lentivirus vector of the invention has the advantages of safety and reliability, long-term expression for the integration of the target genets into the target cell genomes, little immune response, the infection of non-dividing cells and the like and is a kind of ideal vector. The interference effect of the lentivirus vector of the invention on primary culture neurons of rats is over 85 percent, so that the recombined lentivirus vector possibly becomes a powerful means for gene therapy of chronic neuropathic pain.