The invention discloses an antibody capable of resisting heavy metal mercury ion and application thereof. The antibody capable of resisting heavy metal mercury ions is a monoclonal antibody secreted by a hybridoma cell strain H1H8 CCTC No.C 200949. The acquisition of the monoclonal antibody of the metal mercury ion chelate of the invention lays the basis for building the immunological detection method of mercury ion residue. The above monoclonal antibody can be used for researching and building the immunological detection method of heavy metal. The advantages of high speed, low price, agility and strong specificity of the monoclonal antibody are utilized to develop a portable method for on-site detection so as to adapt to the sampling detection of agricultural and animal products and quick detection of import and export custom clearance. Thus, the invention has important realistic significance for improving the efficiency and quality of risk evaluation and ensuring food safety.