The invention discloses a device for dynamically and electrically researching dielectric property of cells by using ultrasonic pulse. The device comprises a computer, an acquisition card and a support with a vertical liquid accommodating cavity; an ultrasonic transducer is arranged in the liquid accommodating cavity of the support; an ultrasonic reflection preventing sound absorption material is filled between the lower end of the ultrasonic transducer and the bottom of the liquid accommodating cavity; a cavity for accommodating a cell suspension sample is positioned above the ultrasonic transducer in the liquid accommodating cavity; a sampling electrode in liquid level contact with the cell suspension sample is arranged at the upper end opening of the liquid accommodating cavity, the sampling electrode is connected with a micro current detection circuit, and the micro current detection circuit is connected to the computer through the acquisition card; and two electrodes of the ultrasonic transducer are connected with ultrasonic signal generating sources. The device can realize research of real-time ultrasonic biological effect under the action of ultrasonic according to a dynamicelectric theoretical model of cell suspension by continuously adjusting the functional state of the ultrasonic; and the device has a simple structure and good repeatability, is easy to operate, and has no damage to the cells.