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发明公开
CN101979664A K88大肠杆菌不同血清型的PCR特异性检测和分型鉴别方法 无效 - 撤回

K88大肠杆菌不同血清型的PCR特异性检测和分型鉴别方法
摘要：
本发明涉及K88大肠杆菌不同血清型的检测方法。该方法是先对待测样本进行PCR特异性检测，出现750bp条带的为待测样本为K88大肠杆菌，再分别用3对特异性引物对K88大肠杆菌DNA进行PCR扩增，根据扩增产物的500bp条带判断血清型。本发明的方法不仅分型结果准确无误，且没有假阳性和相互交叉反应的发生，还具有操作简便，可同时检测多个样品，成本较低的优点。
摘要（英）：

The invention relates to a method for detecting different serotypes of K88 escherichia coli. The method comprises the following steps of: performing polymerase chain reaction (PCR) specificity detection on samples to be detected, wherein the samples to be detected on which 750 bp strips appear are the K88 escherichia coli; performing PCR amplification on K88 escherichia coli deoxyribonucleic acid (DNA) by using 3 pairs of specific primers respectively; and determining the serotypes according to 500 bp strips of amplification products. The method of the invention has the advantages that: classification results are accurate; false positive and mutual cross reactions are not performed; the method is easy and convenient to operate; a plurality of samples can be detected at the same time; and the cost is low.

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