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发明公开
CN102021124A 对虾白斑综合征病毒VP37酵母表面展示与应用 无效 - 撤回

对虾白斑综合征病毒VP37酵母表面展示与应用
摘要：
本发明公开了一种表面展示对虾白斑综合征病毒蛋白VP37的重组酵母菌的制备方法，是利用酵母表面展示载体pYD1，与对虾白斑综合征病毒蛋白VP37基因重组，构建融合表达的重组质粒，并转化酿酒酵母菌EBY100，得到表面展示VP37的重组酵母菌。其特征在于含有对虾白斑综合征病毒蛋白VP37基因的重组酵母菌EBY100，所述的重组酵母菌表面有VP37。本发明的另一个目的是提供表面展示对虾白斑综合征病毒VP37重组酵母活疫苗在甲壳类动物养殖中的应用。所制备的重组酵母菌可以作为口服疫苗免疫对虾，用来保护对虾免受对虾白斑综合征病毒的侵染。本发明的基因工程活疫苗的优点在于，所用酿酒酵母菌安全，培养方法简单易行，便于大规模生产。相对于其它疫苗，展示的VP37蛋白易被免疫系统识别，展示的VP37蛋白稳定性好。
摘要（英）：

The invention discloses a preparation method of recombinant yeast for the surface display of white spot syndrome virus (WSSV) protein VP37, comprising the steps of: constructing a fused expression recombinant plasmid by using a surface display carrier pYD1 of the yeast and the gene recombination of the WSSV protein VP37, and transforming the brewing yeast EBY100 to obtain the recombinant yeast for the surface display of the VP37. The preparation method is characterized in that the recombinant yeast EBY100 containing the WSSV protein VP37 gene has VP37 on the surface. The invention also provides the application of a recombinant yeast live vaccine for the surface display of the WSSV VP37 in the cultivation of crustaceans. The prepared recombinant yeast can be used as an oral vaccine for immunizing the shrimps and protecting the shrimps against the WSSV. The genetic engineering live vaccine in the invention has the advantages that the brewing yeast is safe, and the cultivation method is simple and easy, and convenient for mass production. Relative to other vaccines, the displayed VP37 protein is easy to be identified by an immune system and has good stability.

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