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发明公开
CN102023209A 使用胶乳粒子的免疫测定方法 失效 - 权利终止

使用胶乳粒子的免疫测定方法
摘要：
本发明的一个目的是提供具有高生产再现性的胶乳粒子，通过其可以在免疫诊断中实现足够高的信号ΔS值(＝信号值-噪声值)。本发明提供了一种测定方法，其包括以下步骤：(1)使待测物质与结合物质标记的荧光胶乳粒子接触或竞争，所述结合物质标记的荧光胶乳粒子通过使结合物质与荧光胶乳粒子结合而获得，以及(2)测量来自结合物质标记的荧光胶乳粒子的荧光，其中：(a)所述荧光胶乳粒子是通过将荧光物质加入至胶乳粒子而获得，所述胶乳粒子的特征在于所述胶乳粒子的表面上存在的COOH基团的量为40μeq/g至300μeq/g，或(b)所述荧光胶乳粒子包含胶乳粒子和荧光物质，其特征在于所述荧光胶乳粒子的表面上存在的COOH基团的量为40μeq/g至300μeq/g。
摘要（英）：

An object of the present invention is to provide latex particles with high production reproducibility, by which a sufficiently high value of signal [Delta]S (=value of signal-value of noise) can be realized in immunodiagnosis. The present invention provides a method for assay which comprises steps of (1) causing a substance to be detected to come into contact with or compete with binding substance-labeled fluorescent latex particles which are obtained by causing a binding substance to bind to fluorescent latex particles, and (2) measuring fluorescence from the binding substance-labeled fluorescent latex particles, wherein: (a) the fluorescent latex particles are obtained by adding a fluorescent substance to latex particles characterized in that the amount of COOH groups existing on the surfaces of the latex particles is from 40 [mu]eq/g to 300 [mu]eq/g, or (b) the fluorescent latex particles contain latex particles and a fluorescent substance, which are characterized in that the amount of COOH groups existing on the surfaces of the fluorescent latex particles is from 40 [mu]eq/g to 300 [mu]eq/g.

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