The embodiment provides a method for estimating channels in an MIMO-OFDM (Multiple Input Multiple Output-Orthogonal Frequency Division Multiplexing) system, which comprises the following steps of: receiving data sent by a dual-transmitting antenna OFDM system by using receiving equipment; interpolating discrete pilot frequency data of adjacent symbols according to a pilot frequency configuration mode, and carrying out frequency/time domain conversion on the interpolated discrete pilot frequency data; and separating the data after frequency/time domain conversion to obtain channel information of signals transmitted from different transmitting antennas. The technical scheme provided by the invention solves the problem of the inconvenience that the receiving antenna estimates a channel by using the received data after the receiving antenna receives signals generated by channel superposition of the transmitting signals from two transmitting antennas, and after the pilot frequency information is reasonably configured by the transmitting terminal, each receiving antenna can use the pilot frequency information to obtain the information of the channel that the signals from each transmitting terminal pass through.