The invention relates to a preparation method of gold nanotube material and the application thereof, belonging to the technical field of material preparation. The preparation method of the gold nanotube material comprises the steps of: (1) preparation of a silver nano-wire; (2) preparation of a gold nanotube; and (3) preparation of an electrode actually applied by the gold nanotube. In the preparation process of the gold nanotube, the silver nano-wire is synthesized by a polyol method and then reacts with a gold source by a sacrificing template method after purification and separation, so that the gold nanotube can be prepared. The prepared gold nanotube is better in electric conduction capability and can be further used for catalyst material and the fields of analysis and detection.