The invention provides Ivermectin mixed micelle injection for reducing poisoning incidence and local reaction probability during the killing of livestock parasites and a preparation method thereof. The micelle injection is prepared by taking Ivermectin as active ingredients and adopting a cholate/phosphatide mixed micelle system, so the problem that the Ivermectin is difficult to dissolve in water is solved effectively, the medicine-carrying dose of the micelle injection is improved greatly. The mixed micelle injection prepared by the method has a simple preparation process, and can reduce the probability of toxic and side reactions during the killing of the livestock parasites.