The invention relates to a cold-system preparation method of south calcite as a Tibetan medicine. The method comprises the following steps of: (1) collecting mineral south calcite, cleaning, removing impurities, scrapping into particles with the granularity of 10-30 mm, adding auxiliaries, i.e., Radix Aconiti and potassium nitrate, heating and continuously boiling for 2-16 h to obtain the boiled south calcite; (2) rinsing the boiled south calcite with clear water until the rinsing liquid is clear as crystal, and drying until the weight is consistent to obtain the dry south calcite; and (3) grinding the dry south calcite, sieving with a screen of 20-100 meshes to obtain south calcite powder, uniformly mixing and stirring the south calcite powder with fresh milk and drying in shade to obtain a finished product. The cold-system preparation method is simple and convenient to operate and easy to implement and can greatly increase the content of effective trace elements in the south calcite without remarkably influencing the main contents, thereby the method can be used as a reference for preparing high south calcite and a standard preparation method of the south calcite as the Tibetan medicine.