The invention relates to a method for preparing a high-purity aluminum-vanadium intermediate alloy, which comprises the following steps of: smelting an aluminum-vanadium alloy with not less than 50 percent of vanadium, and high-purity aluminum liquid with over 99.99 percent of Al serving as raw materials, uniformly injecting a refining agent for an aluminum melt into a melt through a powder injection refining machine by taking nitrogen or argon as a carrier in the smelting process, and refining and deslagging, wherein the flow rate of the nitrogen or the argon is 10 to 35NL/min, gas pressure is 0.1 to 0.3MPa, 2 to 6kg of refining agent for the aluminum melt is used for each ton of aluminum, the temperature is controlled to be between 800 and 1,100 DEG C in the production process, and a covering agent for the aluminum melt of potassium salt or sodium salt with the thickness of 1 to 3mm is scattered on the surface of molten aluminum. The content of impurity elements in the obtained high-purity aluminum-vanadium intermediate alloy is not more than 0.03 percent; the content of a main element V is 3.9 to 4.1 percent; the segregation degree of the melt is low; the content of the impurity elements is low and uniform; and the method is particularly favorable for the production of a high-purity aluminum alloy.