The invention relates to the technical field of biology, in particular to a seed-specific promoter sequence separated from soybean and applications thereof. The nucleotide sequence is shown in SEQ No.1. The seed-specific promoter sequence is used as the seed-specific promoter of soybean. In the invention, the stearic acid-acyl carrier protein (ACP) desaturase promoter of soybean is cloned and the tissue expression characteristics and expression efficiency of the promoter in soybean are researched, thus an evidence for the effective use of the promoter is provided; and the promoter provides a good tool for the transgenic research of soybean and creates conditions for the researches and developments by utilizing the soybean seed as bioreactor.