利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN102140555A 一种豇豆花叶病毒属和蚕豆病毒属病毒通用RT-PCR检测用简并引物、检测方法及其应用 失效 - 权利终止

一种豇豆花叶病毒属和蚕豆病毒属病毒通用RT-PCR检测用简并引物、检测方法及其应用
摘要：
本发明公开了一种豇豆花叶病毒属和蚕豆病毒属病毒通用RT-PCR检测用简并引物、检测方法及其应用，由正向和反向引物组成，其正向引物的核苷酸序列见SEQIDNo.1：5′-TGYGAYTAYDVHWSWTTYGATGG-3′；反向引物的核苷酸序列见SEQIDNo.2：5′-AKYARRTTRTCATCHCCATA-3′；其中Y=C/T，D=G/A/T，V=G/A/C，H=A/T/C，W=A/T，S=G/C，K=G/T，R=A/G。本发明检测方法能够快速准确地判断样品是否有豇豆花叶病毒属和蚕豆病毒属病毒，根据序列进行种类鉴定，为进出口安全提供了保证。
摘要（英）：

The invention discloses degenerate primers for universal reverse transcription-polymerase chain reaction (RT-PCR) detection of cowpea mosaic virus and fabavirus, a detection method and application thereof. The degenerate primers consist of a positive primer and a negative primer; the nucleotide sequence of the positive primer is shown as SEQIDNo.1:5'-TGYGAYTAYDVHWSWTTYGATGG-3'; and the nucleotide sequence of the negative primer is shown as SEQIDNo.2: 5'-AKYARRTTRTCATCHCCATA-3', wherein Y=C/T, D=G/A/T, V=G/A/C, H=A/T/C, W=A/T, S=G/C, K=G/T and R=A/G. The detection method can quickly and accurately judge whether a sample contains the cowpea mosaic virus and fabavirus, variety identification is carried out according to a sequence, and guarantee for import and export safety is provided.

公开/授权文献
0/0