The invention discloses a method for detecting diethylstilbestrol in livestock and poultry meat, which comprises the steps of: detecting a standard Raman spectrum of diethylstilbestrol, preparing an extract of a livestock and poultry meat sample, preparing extracts of other samples, detecting and analyzing the Raman spectrum of the sample, and the like. According to the invention, the standard Raman spectrum of diethylstilbestrol and relevant information are acquired by adopting a confocal microprobe Raman spectrum detecting method and the method for detecting diethylstilbestrol in livestock and poultry meat is established. The method for detecting diethylstilbestrol in livestock and poultry meat is effective and sensitive, can be used for detecting and judging diethylstilbestrol in the sample through comparison and analysis with a characteristic peak of a standard spectrum, and requires a small amount of samples; the method is quick, simple and convenient, and is simple to operate inthe detection process; and the scanning time is several seconds to dozens of seconds, and a result can be obtained through analysis of the spectrum within 10 minutes generally, thus the deficiencies of complicated procedures, high cost, long time and the like in the conventional chemical analysis and operation are avoided.