A 9-hydroxyl-9'-aromatic conjugate substituted fluorene-containing polymer material and preparation and application methods thereof belong to the fields of photoelectric materials and organic information display science and technology, and in particular relates to a 9-hydroxyl-9'-aromatic conjugate substituted fluorene-containing homopolymerized or copolymerized conjugate polymer, a preparation method thereof and application of the material to the field of organic electronics such as organic light emitting display and the like. The material has the following structure which is shown in the specification. The material has the advantages that: (1) the synthesis steps are simple and the conditions are mild; (2) the advantages of high thermal stability, glass transition temperature and the like are kept; and (3) the material shows morphological diversity due to a supramolecular effect and has rich optical characteristic. It can be anticipated that the material will become an organic photoelectric function material with commercial potential.