The invention discloses application of traditional Chinese medicine screwtree root extractives in the preparation of anti-HBV medicament. In the invention, the performance of screwtree root extractives like aqueous extractive, alcohol extractive, and triterpenoid in resisting hepatitis B is studied for the first time. According to results of in-vitro and in-vivo tests, the screwtree root extractives can significantly inhibit HBV, mitigate hepatitis B inflammation, alleviate damage to liver histocyte, and have stable and lasting drug effect and no rebound phenomenon; therefore, the screwtree root extractives are superior to Lamivudine, a western medicine commonly used in clinical practice, and have unique advantages in controlling hepatitis B. The screwtree root extractives provided in theinvention have a very good research and development prospect in the treatment of hepatitis B and can be used to prepare traditional Chinese medicine preparation of various dosage forms like tablets, particulate agents, mixtures, and the like for the treatment of hepatitis B.