The invention belongs to the field of traditional Chinese drug extracting, and relates to a method for extracting eleutheroside. The method comprises the following steps: (1) dissolving acanthopanax aqueous extract through methanol, then carrying out a ultrasonic process to uniformly dissolve the acanthopanax aqueous extract, followed by filtering through a micropore film to obtain filtrate to provide a spare use; (2) adopting a macroporous resin column chromatography for separating the filtrate prepared from the step (1), wherein a stationary phase is a HPD-450 macroporous adsorptive resin, a elution solvent 1 is a ethanol water solution having a volume percentage less than 30%, a elution solvent 2 is a ethanol water solution having a volume percentage more than 30%; sequentially adopting 3-6 times of column volume of the elution solvent 1 and the elution solvent 2 to carrying out elutions, and collecting eluent corresponding to the elution solvent 2. With the present invention, impurities are successfully separated from the eleutheroside, a content of the final extracted eleutheroside is high, and industrial cost for preparing the eleutheroside is low.