The invention discloses a pollen separator comprising a support base and a support rod, wherein the support base is provided with a power suction unit and a straight cylinder; the bottom end of the straight cylinder is provided with a straight cylinder bottom end plugging plate; the side wall at the lower part of the straight cylinder is provided with an anther inlet; a screening tube for screening pollen from anther is arranged in the straight cylinder; the screening tube comprises a screen supporting bracket and a screen I wound on the screen supporting bracket; the top end of the straight cylinder is provided with a straight cylinder top end plugging plate; the straight cylinder is arranged at one side of the support rod, and a tapered barrel is arranged at the other side of the support rod; the straight cylinder and the tapered barrel are communicated through a connector; the bottom end of the tapered barrel is provided with a pollen collector; and the power suction unit comprisesa fan I, an electric motor I, a frequency converter I and an electric control system I, wherein the fan I is connected with the top end of the tapered barrel through an exhaust tube I. The pollen separator has reasonable design, high pollen separation efficiency, reliable performance and a good use effect, can effectively ensure the activity of pollen, reduces the production cost and is convenient to popularize and use.