The invention relates to a Chinese and western medicinal compound preparation for preventing and treating bullfrog streptococcus agalactiae diseases and a preparation method, and belongs to the technical field of fish medicines. The Chinese and western medicinal compound preparation is characterized by consisting of the following substances in percentage by weight: 15 to 25 percent of Chinese gall, 5 to 15 percent of common andrographis herb, 10 to 20 percent of rhubarb, 10 to 20 percent of honeysuckle flower, 5 to 15 percent of neomycin sulfate and 25 to 35 percent of starch. The Chinese andwestern medicinal compound preparation is prepared by combining the neomycin sulfate which has a treatment effect on germs and serves as a western medicine, the Chinese gall, the common andrographis herb, the rhubarb and the honeysuckle flower which serve as Chinese medicines and the starch serving as an auxiliary material, has obvious synergistic effect and high curative effect, lasting medicinal effect and low toxicity to the environment and can arouse quick response, and the tolerance is difficult to generate; and the preparation process is simple, easy to realize, safe and convenient to use, popularize and apply.