The invention belongs to the technical field of preparing by-product from oil materials, and specifically, relates to a production technology of sesame protein powder. The production technology adopts sesame as a raw material and comprises the following steps: (1) cleaning and peeling sesame; (2) carrying out low-temperature squeezing of the peeled sesame obtained from step (1) at a temperature of room temperature to 70 DEG C; (3) carrying out extraction deoiling of sesame cakes obtained by the low-temperature squeezing; and (4) carrying out desugaring of the sesame cakes obtained by the step(3). The sesame protein powder prepared by the production technology has the advantages of high product additional values, high purity, and low denaturation rate. The production technology can be widely used in the fields of medicines, foods, feed, chemical industry and the like.