The invention relates to an oligonucleotides primer for detecting apple-derived materials in a sample, and also relates to a realtime fluorescence PCR (Polymerase Chain Reaction) detection method for detecting the apple-derived materials in the sample, wherein the method comprises the step of using the specific oligonucleotides primer aiming at the apple-derived materials in the sample. The invention also relates to a PCR detection kit for quickly detecting the apple-derived materials in the sample, wherein the kit comprises the specific oligonucleotides primer for detecting the apple-derivedmaterials in the sample by using the realtime fluorescence PCR detection method. In addition, the invention relates to application of the specific oligonucleotides primer aiming at the apple-derived materials in the sample in detecting the apple-derived materials in the sample. Due to the use of the PCR detection method and the PCR detection kit, whether the apple-derived materials are contained in the samples such as fruit juice, food, drug, nourishment and the like can be simply, quickly, specifically and sensitively detected.