The invention provides a method for determining a starch content of cane juice or syrup. The method comprises steps of: 1) heating to boil the cane juice or the syrup and carrying out a pumping filtration to obtain a cane juice or syrup clear liquid; 2) adding high temperature amylase into the clear liquid from step 1) and reacting in a water-bath; 3) adding hydrochloric acid and the clear liquidfrom step 2) in series comparison tubes and mixing well; 4) then adding series starch solutions with different volumes into the series comparison tubes; 5) adding iodine solutions into the series comparison tubes respectively; 6) determining starch absorbances of series mixtures obtained in the step 5) and drafting a working curve of the absorbance and the starch content; 7) adding hydrochloric acid and iodine liquid into the clear liquid from step 1) and determining a starch content according to the working curve obtained from step 6). The determination method of the present invention is rapid, accurate, with small error and simply operated.