A detector for biochip fluorescent microspectrum and a manufacture method thereof. (1) represents an upper cover chip, and (2) represents a lower loading chip; (11) represents a micro channel laden with biomicrofluidic to be detected; an upper side and a lower side of the micro channel (11) are equipped with optical excitation units (12) (13), and optical detection units (14) (15) are symmetrically distributed on the left and the right of the micro channel (11); light emitted from an excitation light source (3) is filtered by optical filters (4), and selected excitation light is focalized through optical microlenses (5) at tops of the excitation units and transmits into the micro channel (11) to irradiate the biomicrofluidic to be detected; the substance to be detected is excitated by theexciting light to radiate fluorescence, which is acquired by optical microlenses (7) tops of the detection units; fluorescence filtered through the optical filters is received by semiconductor photoelectric converters and changed into electric signals to be output. The invention combines all non-electrical factors of the spectrum detection; and the detector has a minimal structure to realize micromation of the chip detection apparatus.