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发明公开
CN102367486A 草鱼出血病的病原草鱼呼肠孤病毒RT-PCR快速检测试剂盒及检测方法 无效 - 撤回

草鱼出血病的病原草鱼呼肠孤病毒RT-PCR快速检测试剂盒及检测方法
摘要：
本发明涉及草鱼出血病的病原草鱼呼肠孤病毒RT-PCR快速检测试剂盒及检测方法。本发明所述的草鱼出血病的病原草鱼呼肠孤病毒的RT-PCR快速检测试剂盒，包括：2×反应混合缓冲液1.0ml，包含优选设计的上游引物和下游引物；酶混合液；无RNA酶的去离子水；阳性对照液；阴性对照液。本发明克服了现有草鱼出血病RT-PCR检测方法无法检测当前流行毒株的不足，提供了一种新型的RT-PCR快速检测试剂盒及其检测方法，能快速、高效地适用于目前多数流行毒株的检测。
摘要（英）：

The invention relates to a reverse transcription-polymerase chain reaction (RT-PCR) fast detection reagent kit and a detection method for grass carp hemorrhage disease pathogeny of grass carp reovirus. The RT-PCR fast detection reagent kit for grass carp hemorrhage disease pathogeny of grass carp reovirus comprises 1.0ml of 2* reaction mixed buffer liquid, optimally designed upstream primers and downstream primers, enzyme mixed liquid, deionized water without ribonucleic acid (RNA) enzymes, positive control liquid and negative control liquid. The invention provides the novel RT-PCR fast detection reagent kit and the detection method for grass carp hemorrhage disease pathogeny of grass carp reovirus, which can be fast and efficiently applicable to the detection of most circulating strains in the prior art and overcome the defect that the existing RT-PCR detection method for grass carp hemorrhage disease can not be used for detecting the current circulating strains.

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