The invention discloses a magnetic particle chemiluminescence assay kit for detecting angiotensin II. The kit comprises magnetic particle suspension coupled with anti-rabbit angiotensin II antibody, a calibrator, angiotensin II antigen coupled with biotin, horse radish peroxidase labeled streptavidin, enhanced chemiluminescent A, enhanced chemiluminescent B and lotion. The invention has the advantages that: the kit can be operated conveniently and automatically, a detection result is high in repeatability and accuracy, detection time is short, the stability can be kept for twelve months, and radio contamination can be avoided. During testing, a sample is introduced by a one-step method, namely the magnetic particle suspension, a sample to be tested, the angiotensin II antigen coupled with biotin, and the horse radish peroxidase labeled streptavidin which take part in reaction are added into a reaction cup in one operation step, so operation steps and reaction time are reduced, and errors brought in an operation process are reduced.