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CN102549008A 制备植物来源的蛋白质的方法 无效 - 驳回

制备植物来源的蛋白质的方法
摘要：
提供了制备植物来源之蛋白质或超结构(suprastructure)蛋白的方法。该方法可包括获取包含定位于质外体(apoplast)之蛋白质或超结构蛋白的植物或植物物质，从所述植物或植物物质产生原生质体(protoplast)/原生质球(spheroplast)级分和质外体级分，以及回收质外体级分。质外体级分包含植物来源的蛋白质或超结构蛋白。或者，可以通过用细胞壁降解酶组合物消化植物物质来产生消化级分，从而从包含植物来源之蛋白质或超结构蛋白的植物或植物物质获得蛋白质或超结构蛋白。过滤消化级分以产生过滤级分，从过滤级分中回收植物来源的蛋白质或超结构蛋白。
摘要（英）：

Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins,. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.

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