The invention provides a method for detecting the content of docosahexaenoic acid in egg oil, which belongs to the technical field of functional factor detection methods and comprises saponifing an egg oil component till oil drops disappear at constant temperature after the egg oil component is extracted, performing methyl esterification on the egg oil component through BF3 CH3OH solution, extracting fatty acid methyl ester mixture through hexyl hydride, and diluting and filtering the mixture; detecting the peak area y of docosahexenoic acid methyl ester by jointly adopting gas phase color spectrum and a flame ionization detector; and obtaining the concentration of docosahexaenoic acid in the egg oil by means of equation of linear regression and data processing. The method for detecting the content of docosahexaenoic acid in the egg oil greatly advances the peak time of docosahexenoic acid methyl ester by improving flow speed in a range column pressure can bear and ensures the stability of peak time and the symmetry of the peak shape. Methodology verifying tests improve that the method for detecting the content of docosahexaenoic acid in the egg oil is good in precision, repeatability and stability, low in detection limit and high in accuracy.