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发明授权
CN102634577B 甲型副伤寒沙门菌的逆转录荧光PCR检测用引物 有权

甲型副伤寒沙门菌的逆转录荧光PCR检测用引物
摘要：
本发明提供一种甲型副伤寒沙门菌的逆转录荧光PCR检测用引物，其包括5’-CCTGTTAGAAGTGTTTACAACTT-3’和5’-GCTAAACCACCAAATTGTGT-3’。本发明还提供含有所述引物的用于检测甲型副伤寒沙门菌的试剂盒。本发明是针对甲型副伤寒沙门菌hsdM基因设计的特异性引物，用于检测该靶基因的逆转录荧光PCR反应中，利用该方法检测44株甲型副伤寒沙门菌均呈阳性，其余34种非伤寒沙门菌血清型、致腹泻的其他5种肠道致病菌以及发热为主要症状的8种常见非沙门菌扩增结果均为阴性，说明该方法的特异性、敏感性均较高，逆转录荧光PCR的最低检测限为5pg/反应。
摘要（英）：

The invention provides a primer for detecting reverse transcription fluorescence PCR (polymerase chain reaction) of salmonella paratyphi A, which comprises 5'-CCTGTTAGAAGTGTTTACAACTT-3' and 5'-GCTAAACCACCAAATTGTGT-3'. The invention further provides a kit which contains the primer and is used for detecting the salmonella paratyphi A. The primer is a specificity primer which is designed for hsdM genes of the salmonella paratyphi A. In the reverse transcription fluorescence PCR for detecting the target genes, 44 types of salmonella paratyphi A detected by the method are positive, amplification results of other 34 types of non-salmonella typhi blood serum, other 5 types of pathogenic entero bacteria causing diarrhea and 8 types of common non-salmonella mainly causing fever are negative, accordingly, the method is high in specificity and sensitivity, and lower limit of detection of the reverse transcription fluorescence PCR is 5pg/reaction.

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