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发明公开
CN102653723A 对虾白斑综合征病毒VP28酵母表面展示与应用 无效 - 撤回

对虾白斑综合征病毒VP28酵母表面展示与应用
摘要：
本发明公开了一种表面展示对虾白斑综合征病毒蛋白VP28的重组酵母菌的制备方法，是利用酵母表面展示载体pYD1，与对虾白斑综合征病毒蛋白VP28基因重组，构建融合表达的重组质粒，并转化酿酒酵母菌EBY100，得到表面展示VP28的重组酵母菌。其特征在于含有对虾白斑综合征病毒蛋白VP28基因的重组酵母菌EBY100，所述的重组酵母菌表面有VP28。本发明的另一个目的是提供表面展示对虾白斑综合征病毒VP28重组酵母活疫苗在甲壳类动物养殖中的应用。所制备的重组酵母菌可以作为口服疫苗免疫对虾，用来保护对虾免受对虾白斑综合征病毒的侵染。本发明的基因工程活疫苗的优点在于，所用酿酒酵母菌安全，培养方法简单易行，便于大规模生产。相对于其它疫苗，展示的VP28蛋白易被免疫系统识别，展示的VP28蛋白稳定性好。
摘要（英）：

The invention discloses a preparation method of a recombinant yeast with surface display of prawn white spot syndrome virus VP28, and the recombinant yeast with surface display of VP28 is obtained by performing recombination of a yeast surface display carrier pYD1 with a prawn white spot syndrome virus protein VP28 gene to construct a recombinant plasmid with fusion expression, and transforming saccharomyces cerevisiae EBY100. The invention is characterized in that the recombinant yeast EBY100 containing the prawn white spot syndrome virus protein VP28 gene has VP28 on the surface. Another purpose of the invention is to provide an application of a recombinant yeast live vaccine with surface display of the prawn white spot syndrome virus VP28 in culture of shell-fish animals. The prepared recombinant yeast can be used as an oral vaccine for prawn immunization, and can be used for protecting prawns from being infected by prawn white spot syndrome viruses. The advantages of the gene engineering live vaccine in the invention are that the used saccharomyces cerevisiae is safe; the culture method is simple and practical, and convenient for large-scale production. With respect to other vaccines, the displayed VP28 protein is easy to be recognized by the immune system, and the displayed VP28 protein has good stability.

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