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发明公开
CN102653796A 一种分析啤酒大麦或大麦麦芽微生物群落的方法 无效 - 撤回

一种分析啤酒大麦或大麦麦芽微生物群落的方法
摘要：
本发明公开了一种嵌套聚合酶链式反应-变性梯度凝胶电泳（Nested PCR-DGGE）分析啤酒大麦或大麦麦芽中微生物群落结构的方法，该方法属于微生物生态学技术领域。本发明利用土壤DNA抽提试剂盒提取啤酒大麦、大麦麦芽样品的基因组DNA,采用嵌套PCR扩增细菌16S rDNA、真菌18S rDNA序列，PCR产物经DGGE分析后，对主要条带进行割胶回收，再次PCR后琼脂糖胶回收，T-A克隆，测序分析获取微生物的相关信息。本发明方便、快捷、重复性好，对分析啤酒大麦或大麦麦芽中的微生物及微生物对麦芽品质的影响具有很好的指导意义。
摘要（英）：

The invention discloses a method for analyzing microbial community structures in malting barley or barley malt by adopting nested polymerase chain reaction-denaturing gradient gel electrophoresis (nested PCR-DGGE). The method belongs to the technical field of microbial ecology. The method is characterized by utilizing a soil DNA extraction kit to extract genome DNA of malting barley or barley malt samples, adopting nested PCR to amplify sequences of a bacterium 16S rDNA and a fungus 18S rDNA, after analyzing the PCR products through DGGE, tapping and recycling the main stripes, recycling agarose gel after secondary PCR and carrying out T-A cloning and sequencing analysis to obtain the relevant information of the microbes. The method has the advantages of convenience, quickness and good repeatability and has good guiding significance in analyzing the microbes in the malting barley or barley malt and impacts of the microbes on the malt quality.

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