The invention discloses a method for preparing a human urinary kallidinogenase crude product. The method for preparing the human urinary kallidinogenase crude product comprises the following steps of: after adsorbing urokinase protein in urine for a certain time by using an adsorbing agent, collecting the adsorbing agent in which the urokinase protein is adsorbed and centrally eluting; enabling the eluted urokinase protein solution to pass through a metal chelating affinity chromatography column, wherein a human urinary kallidinogenase inhibiting agent is not adsorbed and the human urinary kallidinogenase is combined onto the metal chelating affinity chromatography column, and the human urinary kallidinogenase inhibiting agent is separated from the human urinary kallidinogenase; and collecting an eluting peak to prepare the human urinary kallidinogenase crude product. Due to the adoption of the technical scheme, the human urinary kallidinogenase is efficiently separated from the humanurinary kallidinogenase inhibition agent in the urine, the subsequent purification treatment for the human urinary kallidinogenase and the human urinary kallidinogenase inhibiting agent is facilitated and the purification yield is increased; and the co-generation of two protein crude products is realized, and thus lower production cost is realized.