利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN102660651A 用于检测链霉菌two-domain漆酶基因的简并引物及其检测方法 无效 - 撤回

用于检测链霉菌two-domain漆酶基因的简并引物及其检测方法
摘要：
本发明用于一种用于检测链霉菌two-domain漆酶基因的简并引物及检测方法，简并引物包括以下上、下游引物：Cu2SF：5’-TACTGGYACTACCACGACCAY-3’；Cu4SR：5’-RTGVSWCTGSACRTGRCAGTG-3’。本发明的检测方法包括以下步骤：以待测样品中的DNA为模板，利用简并引物进行PCR扩增，反应后进行琼脂糖凝胶电泳检测，根据电泳图谱中是否含有380～430bp的单一条带进行初筛；再将初筛回收后的DNA片段克隆测序，将该DNA序列置入核酸数据库中比对，确定待测样品中是否含有链霉菌two-domain漆酶基因。本发明能够快速、准确、特异性地检测链霉菌two-domain漆酶基因。
摘要（英）：

The invention discloses a degenerate primer for detecting a streptomyces two-domain laccase gene and the detection method of the degrnerate primer. The degenerate primer comprises the following upstream and downstream primers: Cu2SF:5'-TACTGGYACTACCACGACCAY-3'and Cu4SR:5'-RTGVSWCTGSACRTGRCAGTG-3'. The detection method comprises the following steps of: carrying out PCR (polymerase chain reaction) amplification by utilizing the degenerate primer based on DNA (deoxyribonucleic acid) in a sample to be detected as a template; after the reaction, carrying out agarose gel electrophoresis detection and preliminary screening according to the fact that whether an electrophoretogram contains a 380-430bp single strip; cloning and sequencing DNA segments which are recovered after the preliminary screening; and putting the DNA sequence in a nucleic acid database for sequence alignment to determine whether the sample to be detected contains the streptomyces two-domain laccase gene. By adopting the detection method, the streptomyces two-domain laccase gene can be rapidly, accurately and specifically detected.

0/0