The invention discloses a camptothecin-containing external bacteriostatic medicine and a preparation method and application thereof, belonging to the technical field of external preparations of traditional Chinese medicines. The camptothecin-containing external bacteriostatic medicine is characterized by comprising the following substances in parts by weight: 8-17 parts of camptothecin, 3-11 parts of leech, 6-15 parts of houttuynia cordata, 5-13 parts of scorpio, 8-15 parts of forsythia, 5-12 parts of hippocampus, 3-9 parts of eupolyphaga, 5-12 parts of herba artemisiae scopariae, 4-11 parts of tortoise plastron, 3-8 parts of borneol, 8-17 parts of beewax and 10-20 parts of medical alcohol. The camptothecin-containing external bacteriostatic medicine and the preparation method and application thereof disclosed by the invention have the functions of inhibiting bacteria and reliving itching, relieving pain and dispelling tinea, removing toxin and drying dampness, preventing inflammation and diminishing swelling, improving microcirculation at the wound and the like, realize an obvious curative effect on the pruritus sore pain symptoms such as tinea corporis, tinea cruris, tinea manus and pedis, tinea versicolor, dermatophytids and the like, and ensure high recovery rate and little toxic side effect; and moreover, the preparation method is simple, safe and effective, and the production cost is low.