The invention discloses a shaft-rotation-resisting stocker gap adjusting mechanism and belongs to the technical field of transportation. The shaft-rotation-resisting stocker gap adjusting mechanism comprises an eccentric shaft penetrating in a frame, a rolling bearing is sleeved at one end of the eccentric shaft to serve as a guide wheel, an adjustment sleeve is arranged on the eccentric shaft and between the guide wheel and the frame, a fixed plate is arranged at the other end of the eccentric shaft, the fixed plate is fixed on the frame through a fixed screw, and a fixed nut is installed on the eccentric shaft on the outside of the fixed plate to enable the eccentric shaft to be assembled on the frame. A guide wheel structure of a shaft-rotation-resisting stocker is simple in structure and convenient to adjust and install, and the fixed plate is added to effectively prevent the fixed nut form loosening due to the fact that the eccentric shaft rotates.