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发明公开
CN102676506A 单核细胞增生李斯特氏菌核酸标准样品、其建立方法及应用 无效 - 撤回

单核细胞增生李斯特氏菌核酸标准样品、其建立方法及应用
摘要：
本发明公开一种单核细胞增生李斯特氏菌（Listeria monocytogenes）核酸标准样品、其建立方法及应用，其通过菌株培养、鉴定、核酸标准样品的制备、核酸标准样品的干燥、均匀性和稳定性检查、定性检定、核酸标准样品定值等项工作,获得一种单核细胞增生李斯特氏菌（Listeria monocytogenes）核酸标准样品。本发明解决了国内食品中致病菌核酸标准样品紧缺的状况，对解决食品中致病菌核酸标准样品的制备技术和稳定性保证技术，积极开展我国食品中致病菌分子生物学检测标准样品的研制，添补该测量领域的空白，具有很重要的现实意义，并对开展食品中致病菌分子生物学检测工作，食品致病菌核酸标准样品具有广阔的市场，有较大的经济效益和社会效益。
摘要（英）：

The invention discloses a mononuclear cell proliferation listeria monocytogenes nucleic acid standard sample and an establishing method and application thereof. The mononuclear cell proliferation listeria monocytogenes nucleic acid standard sample is prepared by the steps of bacterial strain cultivation and identification, preparation of a nucleic acid standard sample, drying of the nucleic acid standard sample, inspection of uniformity and stability, qualitative verification, valuing of the nucleic acid standard sample and the like. The situation of shortage in nucleic acid standard samples of pathogenic bacteria in domestic foods is solved. The mononuclear cell proliferation listeria monocytogenes nucleic acid standard sample, the establishing method and the application have very important practical significance on solving of a preparative technology of the nucleic acid standard samples of the pathogenic bacteria in the foods and a stability guarantee technology, active implementation of development of molecular biological test standard samples of the pathogenic bacteria in the domestic foods, filling of the blank in the measurement field and the implementation of molecular biological detection work of the pathogenic bacteria in the foods. The nucleic acid standard samples of the pathogenic bacteria in the foods have wide market and large economic benefit and social benefit.

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