The invention discloses an amino acid derivative containing a dinitro-trifluoromethylbenzene structure and a preparation method and application of the amino acid derivative, belonging to the technical field of organic chemical synthesis. The method comprises the steps of taking amino acid and 3,5-dinitro-4-chlorine-trifluorotoluene as raw materials and dispersing the raw materials into a solvent, and taking alkali as a catalyst to obtain the amino acid derivative containing the dinitro-trifluoromethylbenzene structure under a heating condition, wherein the compound is novel in structure and has good activity for resisting tomato yellow leaf curl virus. The compound is simple in preparation technology, short in reaction mechanism and high in yield.