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CN102703372A 一种高效降解木质纤维素的菌株保藏培养液及其制备方法 失效 - 权利终止

  • 专利标题： 一种高效降解木质纤维素的菌株保藏培养液及其制备方法
  • 专利标题（英）： Preservation culture solution of bacterial strain for efficiently degrading lignocellulose and preparation method of thereof
  • 申请号： CN201210220180.5
    申请日： 2012-06-29
  • 公开(公告)号： CN102703372A
    公开(公告)日： 2012-10-03
  • 发明人: 林海萍 , 周湘 , 毛胜凤
  • 申请人： 浙江农林大学
  • 申请人地址： 浙江省杭州市临安市环城北路88号
  • 专利权人： 浙江农林大学
  • 当前专利权人： 浙江农林大学
  • 当前专利权人地址： 浙江省杭州市临安市环城北路88号
  • 代理机构： 杭州浙科专利事务所
  • 代理商 吴秉中
  • 主分类号： C12N1/38
  • IPC分类号： C12N1/38
一种高效降解木质纤维素的菌株保藏培养液及其制备方法
摘要：
一种高效降解木质纤维素的菌株保藏培养液及其制备方法，属于生物技术领域。其特征在于每1L去离子水中含有下述物质：硫酸镁、硫酸铁、硝酸铵、谷氨酰胺、钼酸钠、氯化钴、碘化钾、磷酸二氢钾、酵母粉、葡萄糖、硫化甘醇酸钠、柠檬酸、淀粉、牛肉膏、天冬酰胺、桃胶。上述一种高效降解木质纤维素的菌株保藏培养液，培养液中添加了多种能够促进降解木质纤维素的菌株迅速、稳定生长的营养元素；其培养液制作方法简便，设备投资少，原料成本低廉，且能准确稳定高效的培养出适应性强的优良菌株，所得菌株降解木质纤维素的活性高于自然界中自然生长的菌株。
摘要（英）：

The invention relates to a preservation culture solution of bacterial strain for efficiently degrading lignocellulose and a preparation method of the culture solution, belonging to the field of biotechnology. The culturing solution provided by the invention is characterized in that each 1L of deionized water contains the following materials: magnesium sulfate, ferric sulfate, ammonium nitrate, glutamine, sodium molybdate, cobalt chloride, potassium iodide, monopotassium phosphate, yeast powder, glucose, sulfuretted sodium glycollate, citric acid, starch, beef extract, asparagine and peach gum. The culturing solution is added with multiple nutrient elements which can promote the bacterial strain for degrading lignocellulose to rapidly and stably grow; the culturing solution is simple in preparation method, low in equipment investment, low in material cost and can accurately, stably and efficiently cultivate excellent bacterial strains with good adaptability; and the lignocellulose degrading activity of the obtained bacterial strain is higher than that of the bacterial strain naturally grown in natural world.

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