The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.