The invention discloses a strawberry seedling culture tray, which comprises a trough body with the rectangular cross section, wherein the rectangular trough body comprises a trough bottom (1), two opposite long trough walls (2) and two opposite short trough walls (3), a plurality of through holes (4) are arranged on the trough bottom, a stripped bulge (6) with a groove (5) is respectively arranged at the upper edge of the two opposite short trough walls, the shape of one bulge is in embedded matching with an inner cavity of the other bulge, and a plurality of trays can be connected into a whole. The strawberry seedling culture tray provided by the invention can be used for the propagation production of strawberry creeping stem seedlings, and the placement and the management are convenient. Experiments prove that the young seedling emergence rate after the use of the strawberry seedling culture tray can reach 93.51 percent to 96.82 percent, the propagating factor can reach 1:39.7-1:77.8, the survival rate of the young transplanted seedlings can reach 96.7 to 98.4 percent, the strong seedling rate of the young transplanted seedlings can reach 87.7 to 93.1 percent, the values are respectively and obviously higher than those of a control group, the obtained young seedlings have the advantages that the flower bud differentiation is early, the seedling recovery is not needed after the fixed planting, the growth conditions are regular, the fruit setting is realized 30 days earlier, the yield is improved, and the economic benefits are obviously improved.