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发明公开
CN102740896A IGF-I聚乙二醇缀合物 有权

IGF-I聚乙二醇缀合物
摘要：
本文报道了制备与一个聚乙二醇缀合的多肽的方法，所述方法包括：a)提供编码表达构建体的核酸，所述核酸在5′至3′方向上包含编码多肽的核酸，以及编码胰蛋白酶位点SEQ ID NO：01的核酸，b)在细胞中表达a)的核酸，并且从细胞和/或培养基回收表达构建体，c)给目标肽提供于C末端赖氨酸残基处与聚乙二醇共价缀合的氨基酸序列SEQ ID NO：02，d)将表达构建体和目标肽与胰蛋白酶突变体D189K，K60E，N143H，E151H温育，并且e)从温育混合物回收并由此产生与一个聚乙二醇缀合的多肽。
摘要（英）：

Herein is reported a method for producing of a polypeptide conjugated to one poly (ethylene glycol) comprising a) providing a nucleic acid encoding an expression construct comprising in 5' to 3' direction a nucleic acid encoding a polypeptide, and a nucleic acid encoding a trypsin site of SEQ ID NO: 01, b) expressing the nucleic acid of a) in a cell and recovering the expression construct from the cell and/or the cultivation medium, c) providing a target peptide with an amino acid sequence of SEQ ID NO: 02 covalently conjugated to a poly (ethylene glycol) at the C-terminal lysine residue, d) incubating the expression construct and the target peptide with the trypsin mutant D189K, K60E, N143H, E151H, and e) recovering and thereby producing the polypeptide conjugated to one poly (ethylene glycol) from the incubation mixture.

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