利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN102766686A 食品和饮料中大蒜成分快速检测试剂盒的制备和检测方法 有权 转让

食品和饮料中大蒜成分快速检测试剂盒的制备和检测方法
摘要：
本发明属于食品和饮料中植物源性成分的定性检测技术，具体地说是用实时荧光PCR技术检测食品和饮料中大蒜成分的试剂盒及方法。试剂盒包括2×PCR Master Mix、上游引物、下游引物、Taqman探针；其中2×PCR Master Mix中含有Taq DNA聚合酶、反应缓冲液、氯化镁、dNTP，上游引物序列为5′-GGAAATGCTGCGAACTATGTGA-3′、下游引物序列为5′-TTGATTGGGCTGTAATGAGGC-3′、TaqMan探针序列为5′-FAM-TGGCAAGGAGACCTTCTGGGTTGTTAGG-TRAMA-3′；大蒜成分的快速检测方法包括食品和饮料DNA的提取、大蒜成分的实时荧光PCR扩增和结果判定。其优点是快速、特异性强、灵敏度高、操作简便、重复性好。
摘要（英）：

The invention belongs to the field of qualitative detection of plant derived ingredients in food and beverage, and specifically provides a kit and a method for detecting a garlic component in food and beverage by a real-time fluorescent PCR detection technology. The kit comprises 2 * PCR Master Mix, an upstream primer, a downstream primer, and a Taqman probe. The 2*PCR Master Mix contains Taq DNApolymerase, a reaction buffer, magnesium chloride, and dNTP; the upstream primer has a sequence of 5'-GGAAATGCTGCGAACTATGTGA-3'; the downstream primer has a sequence of 5'-TTGATTGGGCTGTAATGAGGC-3'; and the Taqman probe has a sequence of 5'-FAM-TGGCAAGGAGACCTTCTGGGTTGTTAGG-TRAMA-3'. The rapid determination method of garlic component comprises steps of: extraction of DNA of food and beverage; real-time fluorescent PCR amplification on the garlic component and result determination. The invention has advantages of fastness, strong specificity, high sensitivity, simple operation and good repeatability.

公开/授权文献
0/0