The invention provides a nursing traditional Chinese medicine disinfectant and a preparation method. The nursing traditional Chinese medicine disinfectant comprises, by weight, 10-20 grams of camphor bark, 5-10 grams of honeysuckle, 8-12 grams of light-yellow sophora root, 5-15 grams of glabrous greenbrier rhizome, 10-15 grams of common cnidium fruit, 4-6 grams of coptis, 3-5 grams of ginger, 4.2-6.5 grams of safflower, 10.5-12g grams of cortex dictamni, 12.4-13 grams of aloe and 7.8-12.8 grams of ginkgo seeds. The preparation method includes the steps: (1) weighting the raw materials by weight; (2) adding water into the raw materials for decocting twice, filtering, mixing filtrates and adding sterile water into the mixture to 1000ml; and (3) adding 5 grams of nonyl phenol polrvinyl chloride ether and 10 grams of glycerol into the mixture with uniformly stirring, and adding 2 grams of available iodine and 2 grams of metronidazole with uniformly stirring. The nursing traditional Chinese medicine disinfectant is good in disinfection effect, low in skin irritation, suitable for disinfecting the skin and capable of disinfecting clothes and tableware.