The invention relates to a regeneration method of a cyclohexene hydration catalyst. The regeneration method includes steps that: molecular sieve catalyst water solution with activity reduced in a cyclohexene hydration reaction is subjected to gas stripping so as to remove entrained organics, the catalyst solution is subjected to oxidization for further oil removing, and the catalyst with oil removed is subjected to filtering for impurity removing and harmonious stabilizing. The activity of the catalyst obtained after the steps is restored in the maximum limit, and the catalyst is directly sent back to a hydration reaction system by a pump for cycle use. The regeneration method of the cyclohexene hydration catalyst enables the catalyst activity to restore to be above 95% of the initial activity, and achieves online regeneration of the catalyst when the catalyst is in the slurry state same as that in a reactor.