The invention belongs to the field of medical and biological technology, and relates to a method for screening an ALK fusion gene, in particular to a mulitsegment primer for screening the ALK fusion gene and application of the method for screening the ALK fusion gene. The method for screening the ALK fusion gene comprises the following steps: taking the inverted cDNA of total RNA in a sample as a template, adding real-time PCR (polymerase chain reaction) combined primer presented by nucleotide coded sequence such as SEQ IDNO1-14, carrying out real-time fluorescent polymerase chain reaction (PCR), and detecting whether the ALK fusion gene exists in a reaction product through detecting the Ct value of reaction units. The method has the characteristics that the operation is simple and convenient, the time is saved, the result judgment is intuitive and clear, the cost is lower and the pollution is reduced.