The invention discloses a silybin proliposomes preparation, and a preparation method and application method thereof, belonging to pharmaceutical technical field. The silybin proliposomes preparation comprises the following materials by weight: 0.8-1% of silybin, 4-17% of phospholipids, 0.22-0.43% of sodium oleate, and the balance of ethyl lactate or 1,2-propylene glycol. The silybin proliposomes preparation effectively applies proliposomes preparation method into silybin extract, to create a new silybin lipid dosage form; utilizes liver targeting characteristic of lipid, to concentrate silybin at liver site after administration, thereby improving silybin curative effect and effectively reducing side effects; and utilizes absorption-promoting effect of lipid, to improve in-vivo bioavailability of silybin compared with silybin material drug, thereby overcoming the defects of existing silybin preparations such as poor absorption and low bioavailability.