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发明公开
CN102925381A 一种产酯酶B1的海洋枯草芽孢杆菌C5及其酯酶B1 失效 - 权利终止 许可

一种产酯酶B1的海洋枯草芽孢杆菌C5及其酯酶B1
摘要：
本发明涉及一种产酯酶B1的海洋枯草芽孢杆菌C5及产酯酶B1,该海洋枯草芽孢杆菌C5基因序列长1462bp,16S rDNA基因序列如图1所示,该酯酶B1最适温度为45℃，最适pH为8.0，温度稳定范围为0～30℃，pH稳定范围为7.0～10.0,其分子量为83.8kDa,金属离子中Na+、Mn2+、Ca2+、Sr2+、Li+、Co2+、Mg2+、K+、Fe2+对酶活没有影响,Al3+、Cu2+、Zn2+能够一定程度的抑制酯酶B1的酶活,Ag+、Hg+、Fe3+对酯酶B1活性抑制,Ba2+离子能够提高酯酶B1的活性，EDTA对酯酶B1活性有抑制作用。该酯酶B1可广泛应用于食品、医药、化工、降解农药及环保等行业。
摘要（英）：

The invention relates to marine bacillus subtilis C5 for producing esterase B1 and esterase B1 thereof. The gene sequence of marine bacillus subtilis C5 is 1462bp in length, and the 16S rDNA (recombinant deoxyribonucleic acid) gene sequence is shown in figure 1; the optimum temperature of esterase B1 is 45 DEG C, the optimum pH is 8.0, the temperature stability range is from 0 to 30 DEG C, the pH stability range is from 7.0 to 10.0, and the molecular weight is 83.8 kDa; and Na<+>, Mn<2+>, Ca<2+>, Sr<2+>, Li<+>, Co<2+>, Mg<2+>, K<+> and Fe<2+> in metal ions have no influence on enzyme activity, Al<3+>, Cu<2+> and Zn<2+> can inhibit the enzyme activity of esterase B1 to a certain extent, Ag<+>, Hg<+> and Fe<3+> inhibit the activity of esterase B1, Ba<2+> ions can enhance the activity of esterase B1, and EDTA (ethylene diamine tetraacetic acid) plays a role of inhibiting the activity of esterase B1. Esterase B1 can be widely applied to the industries of food, medicine, chemistry, pesticide degradation, environmental protection, etc.

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