The invention discloses an LTPS-TFT array substrate and a manufacture method thereof, and a display device, belonging to the field of liquid display. According to the manufacture method of the LTPS-TFT array substrate, one time construction process is adopted, meanwhile a pixel electrode and a semiconductor layer of the array substrate are formed, and the semiconductor layer is made of polycrystalline silicon and Mask doping is reduced. According to the technical scheme, the manufacture process of the LTPS-TFT array substrate can be simplified, and the manufacture cost of the LTPS-TFT array substrate is lowered.