The invention relates to a ectotrophic mycorrhiza mycelium field in situ dynamic monitoring method in ecological environment reconnaissance. The monitoring method can completely monitor mycelium embedded on host plant root systems, and simultaneously in situ monitoring for a long time, and accordingly development rule of mycorrhiza and flora at different growing periods can be dynamically obtained. Therefore, technical support is provided for ectotrophic mycorrhiza and flora action mechanism study, quantitative study and application, and further important actual ecological significance for subsequent land restoration.