A channel depth information generating method based on an electronic navigation chart and water level data includes the following steps: arranging datum plane information of a water depth point on the electronic navigation chart; dynamically correcting water depth value according to measured and forecasted water level information released by a gaging station along a river along with the datum plane information of the electronic navigation chart, and obtaining absolute water depth value; obtaining a fathom line according to the absolute water depth value; calculating a navigable water area with specific depth and a dynamic central line according to depth range of the fathom line, and releasing the generated water depth information through Web Service. Based on a public service platform, network communication environment and a marine terminal application system of a Yangtze River electronic navigation chart system, the channel depth information generating method based on the electronic navigation chart and the water level data is capable of calculating depth information of the Yangtze River main channel and being used by the marine terminal application system in the form of data services.